Article
Enzymes assembled from Aquifex aeolicus and Escherichia coli leucyl-tRNA synthetases.
Biochemistry - 1 Jul 2003
Zhao Ming-Wei, Hao Rui, Chen Jian-Feng, Martin Franck, Eriani Gilbert, Wang En-Duo
Abstract excerpt
Aquifex aeolicus alphabeta-LeuRS is the only known heterodimeric LeuRS, while Escherichia coli LeuRS is a canonical monomeric enzyme. By using the genes encoding A. aeolicus and E. coli LeuRS as PCR templates, the genes encoding the alpha and beta subunits from A. aeolicus alphabeta-LeuRS and the equivalent amino- and carboxy-terminal parts of E. coli LeuRS (identified as alpha' and beta') were amplified and...
Topics
- Amino Acyl-tRNA Synthetases
- Escherichia coli
- Evolution, Molecular
- Gene Transfer, Horizontal
- Hot Temperature
- Kinetics
- Mutation
- RNA, Transfer, Leu
- Structure-Activity Relationship