Article
Characterization of tetracycline-inducible bitransgenic Krt12rtTA/+/tet-O-LacZ mice.
Investigative ophthalmology & visual science - 1 Jun 2005
Chikama Tai-Ichiro, Hayashi Yasuhito, Liu Chia-Yang, Terai Noriko, Terai Kazuto, Kao Candace W-C, Wang Li, Hayashi Miyuki, Nishida Teruo, Sanford Philip, Doestchman Tom, Kao Winston W-Y
Abstract excerpt
PURPOSE: To prepare binary transgenic mouse lines that overexpress reporter genes in a corneal-epithelium-specific manner when induced by doxycycline. METHODS: A gene-targeting construct containing an internal ribosomal entry site-reverse tetracycline transcription activator (IRES-rtTA) cassette was inserted into the Krt12 allele (keratin 12 gene) to produce a knock-in Krt12(rtTA/+) mouse line through...
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