Article
Site-directed mutagenesis study of the role of histidine residues in the neutral-to-basic transition of human serum albumin.
Biochimica et biophysica acta - 20 Jun 2005
Yang Jinsheng, Ha Chung-Eun, Bhagavan Nadhipuram V
Abstract excerpt
Site-directed mutagenesis was used to study the role of histidine residues located in domain I in the neutral-to-basic (N-B) transition of human serum albumin (HSA). Based on a previous study of the N-B transition by means of proton NMR, the following recombinant HSA species were synthesized in the yeast species, Pichia pastoris: H9F, H9S, H39F, H39S, H67F, H67S, H105F, H105S, H128F, H128S, H146F, H146S, and wild...
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