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Article

Domain-specific fluorescence resonance energy transfer (FRET) sensors of metallothionein/thionein.

Protein engineering, design & selection : PEDS - 1 Jun 2005

Hong S-H, Hao Q, Maret W

Abstract excerpt

Each of the two domains of mammalian metallothioneins contains a zinc-thiolate cluster. Employing site-directed mutagenesis and chemical modification, fluorescent probes were introduced into human metallothionein (isoform 2) with minimal perturbations of the structures of these clusters. The resulting FRET (fluorescence resonance energy transfer) sensors are specific for each domain. The design and construction...

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Identifiers

PMID: 15911539
DOI: 10.1093/protein/gzi031

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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