Article
A fluorescence resonance energy transfer sensor for the beta-domain of metallothionein.
Proceedings of the National Academy of Sciences of the United States of America - 4 Mar 2003
Hong Sung-Hye, Maret Wolfgang
Abstract excerpt
We have designed a nanosensor to study the potential function of metallothionein (MT) in metal transfer and its interactions with redox partners and ligands by attaching two fluorescent probes to recombinant human MT. The specific labeling takes advantage of two different modification reactions. One is based on the fact that recombinant MT has a free N-terminal amino group when produced by the IMPACT T7...
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