Article
Engineering dihydropteroate synthase (DHPS) for efficient expression on M13 phage.
Biochimica et biophysica acta - 20 Jun 2005
Brockmann Eeva-Christine, Lamminmäki Urpo, Saviranta Petri
Abstract excerpt
Phage display is a commonly used selection technique in protein engineering, but not all proteins can be expressed on phage. Here, we describe the expression of a cytoplasmic homodimeric enzyme dihydropteroate synthetase (DHPS) on M13 phage, established by protein engineering of DHPS. The strategy included replacement of cysteine residues and screening for periplasmic expression followed by random mutagenesis and...
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