Isolation and phenotypic analysis of conditional-lethal, linker-insertion mutations in the gene encoding the largest subunit of RNA polymerase II in Saccharomyces cerevisiae.

doi:10.1007/BF00266244

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Article

Isolation and phenotypic analysis of conditional-lethal, linker-insertion mutations in the gene encoding the largest subunit of RNA polymerase II in Saccharomyces cerevisiae.

Molecular & general genetics : MGG - 1 Apr 1992

Archambault J, Drebot M A, Stone J C, Friesen J D

Abstract excerpt

Linker-insertion mutagenesis was used to isolate mutations in the Saccharomyces cerevisiae gene encoding the largest subunit of RNA polymerase II (RPO21, also called RPB1). The mutant rpo21 alleles carried on a plamid were introduced into a haploid yeast strain that conditionally expresses RPO21...

Topics

Alleles
Amino Acid Sequence
Base Sequence
DNA, Fungal
Genes, Fungal
Genes, Lethal
Kinetics
Macromolecular Substances
Molecular Sequence Data
Mutagenesis, Insertional
Oligodeoxyribonucleotides

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Plasmids

Promoter Regions, Genetic

RNA Polymerase II

Saccharomyces cerevisiae

Sequence Homology, Nucleic Acid

Source

Open in PubMed

Identifiers

PMID: 1588909
DOI: 10.1007/BF00266244

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1588909-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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