Article
Genotyping of human neutrophil antigen-5a (Ond).
Transfusion medicine (Oxford, England) - 1 Apr 2005
Sachs U J H, Reil A, Bauer C, Bux J, Bein G, Santoso S
Abstract excerpt
Antibodies to human neutrophil alloantigens (HNA) can cause immune-mediated neutropenias and transfusion complications. The diagnosis of antibodies to the HNA-5a (Ond) isoform of the alphaLbeta2 integrin is hampered by the lack of reliable methods for HNA-5a antigen typing. We have devised a polymerase chain reaction sequence-specific primer method (PCR-SSP) and used it to determine the HNA-5a gene frequencies in...
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