Article
Determination of human neutrophil antigen-1, -3, -4 and -5 allele frequencies in English Caucasoid blood donors using a multiplex fluorescent DNA-based assay.
Vox sanguinis - 1 Jul 2013
Cardoso S P, Chong W, Lucas G, Green A, Navarrete C
Abstract excerpt
BACKGROUND AND OBJECTIVES: A number of DNA-based methods to genotype the alleles coding for HNA have been described, but all require the separate amplification and analysis of each allele. The aim was to develop a DNA-based method for simultaneous detection of HNA-1, HNA-3, HNA-4 and HNA-5 alleles. MATERIALS AND METHODS: An allele-specific primer extension method was used in combination with magnetic beads from...
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