A Novel Acid-Stable Endo-Polygalacturonase from Penicillium oxalicum CZ1028: Purification, Characterization, and Application in the Beverage Industry.

Abstract excerpt

Acidic endo-polygalacturonases are the major part of pectinase preparations and extensively applied in the clarification of fruits juice, vegetables extracts, and wines. However, most of the reported fungal endo-polygalacturonases are active and stable under narrow pH range and low temperatures. In this study, an acidic endo-polygalacturonase (EPG4) was purified and characterized from a mutant strain of...

Topics

• Amino Acid Sequence
• Carica
• Cloning, Molecular
• Cold Temperature
• Enzyme Stability
• Fruit and Vegetable Juices
• Hydrogen-Ion Concentration
• Kinetics
• Mutation
• Pectins