Article
A factorial experiment for optimizing the PCR conditions in routine genotyping.
Biotechnology and applied biochemistry - 1 Oct 2005
Niens Marijke, Spijker Geert T, Diepstra Arjan, te Meerman Gerard J
Abstract excerpt
Although most PCRs would produce proper PCR products when first tried, a general optimization is required to yield the best results. This optimization is often achieved by changing one factor at a time. However, this may lead to suboptimal results, since interactions between conditions are difficult to detect with this approach. In the present study, we describe the factorial optimization of PCR conditions for...
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