Article
Experimental design: a useful tool for PCR Optimization.
BioTechniques - 1 Jul 1996
Boleda M D, Briones P, Farrés J, Tyfield L, Pi R
Abstract excerpt
Because of complex interactions among the components of PCR and the wide and increasing variety of applications in which this technique is used, optimization is necessary for every reaction. Here we describe the use of experimental design techniques (2k fractional factorial design and central composite design) to attain easier, quicker and cheaper PCR optimization of DNA from blood spots. By determining the...
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