Article
A high-throughput fluorescence polarization assay specific to the CD4 binding site of HIV-1 glycoproteins based on a fluorescein-labelled CD4 mimic.
The Biochemical journal - 15 Aug 2005
Stricher François, Martin Loïc, Barthe Philippe, Pogenberg Vivian, Mechulam Alain, Menez André, Roumestand Christian, Veas Francisco, Royer Catherine, Vita Claudio
Abstract excerpt
The three-dimensional structure of CD4M33, a mimic of the host-cell receptor-antigen CD4 and a powerful inhibitor of CD4-gp120 (viral envelope glycoprotein 120) interaction and HIV-1 entry into cells [Martin, Stricher, Misse, Sironi, Pugniere, Barthe, Prado-Gotor, Freulon, Magne, Roumestand et al. (2003) Nat. Biotechnol. 21, 71-76], was solved by 1H-NMR and its structure was modelled in its complex with gp120. In...
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