Article
Murine monoclonal antibody which can distinguish cystatins SA1 and SA2.
Molecular immunology - 1 Jun 2005
Ito Taichi, Komiya-Ito Akiyo, Okuda Katsuji, Minaguchi Kiyoshi, Saitoh Eiichi, Yamada Satoru, Kato Tetsuo
Abstract excerpt
To develop a diagnostic trial enabling the selective examination for a target cystatin in human body fluids, we attempted to prepare monoclonal antibodies against human cystatin SA1 (originally cystatin SA) and its variant form (cystatin SA2). BALB/c mice were immunized with recombinant (r-) cystatins SA1 and SA2. Two monoclonal antibodies designated Cys3F11 and Cys2E5 were selected. By ELISA analyses, the Cys2E5...
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