Phosphorothioate substitution identifies phosphate groups important for pre-mRNA splicing.

doi:10.1093/nar/20.8.1949

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Article

Phosphorothioate substitution identifies phosphate groups important for pre-mRNA splicing.

Nucleic acids research - 25 Apr 1992

Maschhoff K L, Padgett R A

Abstract excerpt

Substitution of pre-mRNA in vitro splicing substrates with alpha-phosphorothioate ribonucleotide analogs has multiple effects on the processes of spliceosome formation and splicing. A major effect of substitution is on the splicing cleavage/ligation reactions. Substitution at the 5' splice junction blocks the first cleavage/ligation reaction while substitution at the 3' splice junction blocks the second...

Topics

Adenoviridae
Animals
Base Sequence
Globins
Molecular Sequence Data
Mutation
Phosphates
RNA Precursors
RNA Splicing
RNA, Messenger
Rabbits
Thionucleotides

Viral Proteins

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