Article
Phosphorothioate substitution identifies phosphate groups important for pre-mRNA splicing.
Nucleic acids research - 25 Apr 1992
Maschhoff K L, Padgett R A
Abstract excerpt
Substitution of pre-mRNA in vitro splicing substrates with alpha-phosphorothioate ribonucleotide analogs has multiple effects on the processes of spliceosome formation and splicing. A major effect of substitution is on the splicing cleavage/ligation reactions. Substitution at the 5' splice junction blocks the first cleavage/ligation reaction while substitution at the 3' splice junction blocks the second...
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