Article
Recombineered Xenopus tropicalis BAC expresses a GFP reporter under the control of Arx transcriptional regulatory elements in transgenic Xenopus laevis embryos.
Genesis (New York, N.Y. : 2000) - 1 Apr 2005
Kelly Lisa E, Davy Brian E, Berbari Nicolas F, Robinson Michael L, El-Hodiri Heithem M
Abstract excerpt
The aristaless-related homeobox (Arx) gene is expressed in a dynamic pattern in the developing vertebrate forebrain. We identified a bacterial artificial chromosome (BAC) containing the Xenopus tropicalis Arx gene and replaced a portion of the first coding exon with a green fluorescent protein (GFP) expression cassette by homologous recombination in bacteria (recombineering). Transgenic X. laevis embryos obtained...
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