Article
Myosin Va transports dense core secretory vesicles in pancreatic MIN6 beta-cells.
Molecular biology of the cell - 1 Jun 2005
Varadi Aniko, Tsuboi Takashi, Rutter Guy A
Abstract excerpt
The role of unconventional myosins in neuroendocrine cells is not fully understood, with involvement suggested in the movement of both secretory vesicles and mitochondria. Here, we demonstrate colocalization of myosin Va (MyoVa) with insulin in pancreatic beta-cells and show that MyoVa copurifies with insulin in density gradients and with the vesicle marker phogrin-enhanced green fluorescent protein upon...
Topics
- Animals
- Biological Transport
- Biomarkers
- Cell Culture Techniques
- Cell Line, Tumor
- Cell Membrane
- Centrifugation, Density Gradient
- Exocytosis
- Glucose
- Green Fluorescent Proteins
- Humans
- Immunohistochemistry
- Insulin
- Islets of Langerhans