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Article

The effect of charge-introduction mutations on E. coli thioredoxin stability.

Biophysical chemistry - 1 Apr 2005

Perez-Jimenez Raul, Godoy-Ruiz Raquel, Ibarra-Molero Beatriz, Sanchez-Ruiz Jose M

Abstract excerpt

Technological applications of proteins are often hampered by their low-stability and, consequently, the development of procedures for protein stabilization is of considerable biotechnological interest. Here, we use simple electrostatics to determine positions in E. coli thioredoxin at which mutations that introduce new charged residues are expected to lead to stability enhancement. We also obtain the...

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Identifiers

PMID: 15752590
DOI: 10.1016/j.bpc.2004.12.012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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