Article
Selection of ura5 and ura3 mutants from the two varieties of Cryptococcus neoformans on 5-fluoroorotic acid medium.
Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology - 1 Jan 1992
Kwon-Chung K J, Varma A, Edman J C, Bennett J E
Abstract excerpt
Spontaneous mutants requiring uracil were isolated from both varieties of Cryptococcus neoformans by plating on 5-fluoroorotic acid (5-FOA) medium. Of the 36 strains tested (18 var. neoformans and 18 var. gattii), 24 (12 of each variety) generated 5-FOA-resistant cells requiring uracil for growth...
Topics
- Animals
- Blotting, Southern
- Cryptococcus neoformans
- Culture Media
- DNA, Fungal
- Deoxyribonuclease EcoRI
- Deoxyribonucleases, Type II Site-Specific
- Humans
- Mutation
- Nucleic Acid Hybridization
- Orotic Acid
- Orotidine-5'-Phosphate Decarboxylase
- Restriction Mapping
- Transformation, Genetic
- Uracil
- Uridine