Article
Selection of ura5 and ura3 mutants from the two varieties of Cryptococcus neoformans on 5-fluoroorotic acid medium.
Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology - 1 Jan 1992
Kwon-Chung K J, Varma A, Edman J C, Bennett J E
Abstract excerpt
Spontaneous mutants requiring uracil were isolated from both varieties of Cryptococcus neoformans by plating on 5-fluoroorotic acid (5-FOA) medium. Of the 36 strains tested (18 var. neoformans and 18 var. gattii), 24 (12 of each variety) generated 5-FOA-resistant cells requiring uracil for growth. Six of the 12 C. neoformans var. gattii strains produced ura3 cells while the remaining six strains produced ura5...
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