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Article

Thermostability of ancestral mutants of Caldococcus noboribetus isocitrate dehydrogenase.

FEMS microbiology letters - 15 Feb 2005

Iwabata Hisako, Watanabe Keiko, Ohkuri Takatoshi, Yokobori Shin-Ichi, Yamagishi Akihiko

Abstract excerpt

We constructed mutant genes of Caldococcus noboribetus isocitrate dehydrogenase containing ancestral amino acid residues that were inferred using the maximal likelihood method and a composite phylogenetic tree of isocitrate dehydrogenase and 3-isopropylmalate dehydrogenase. The mutant genes were expressed in Escherichia coli and the protein products purified. Thermostabilities, reported as the half-inactivation...

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PMID: 15686840
DOI: 10.1016/j.femsle.2004.12.030

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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