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Article

Surface density dependence of PCR amplicon hybridization on PNA/DNA probe layers.

Biophysical journal - 1 Apr 2005

Yao Danfeng, Kim Junyoung, Yu Fang, Nielsen Peter E, Sinner Eva-Kathrin, Knoll Wolfgang

Abstract excerpt

Surface plasmon field-enhanced fluorescence spectroscopy was employed to extensively investigate the hybridization behaviors of polymerase chain reaction (PCR) amplicons on a peptide nucleic acid (PNA) or DNA probe layer that was previously attached on a streptavidin-modified gold surface via biotin/streptavidin interaction. Despite the neutral backbone of PNA, the hybridization reactions were strongly influenced...

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Identifiers

PMID: 15665129
DOI: 10.1529/biophysj.104.051656
PMCID: PMC1305370

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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