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Article

Reverse Genetics System for Porcine Enteric Calicivirus, a Prototype Sapovirus in the <i>Caliciviridae</i>

13 Jan 2005

Abstract excerpt

A porcine enteric calicivirus (PEC), strain Cowden in the genus Sapovirus of the Caliciviridae family, can be propagated in a porcine kidney continuous cell line (LLC-PK) in the presence of bile acids in the cell culture medium. A full-length cDNA copy of the Cowden PEC genome was cloned into a plasmid vector directly downstream from the T7 RNA polymerase promoter, and capped RNA transcripts derived from this...

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Identifiers

PMID: 15650167
DOI: 10.1128/jvi.79.3.1409-1416.2005

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