Article
A DNA-launched reverse genetics system for porcine reproductive and respiratory syndrome virus reveals that homodimerization of the nucleocapsid protein is essential for virus infectivity.
Virology - 5 Jan 2005
Lee Changhee, Calvert Jay G, Welch Siao-Kun W, Yoo Dongwan
Abstract excerpt
Reverse genetic systems were developed for a highly virulent 'atypical' porcine reproductive and respiratory syndrome virus (PRRSV). The full-length genome of 15395 nucleotides was assembled as a single cDNA clone and placed under either the prokaryotic T7 or eukaryotic CMV promoter. Transfection of cells with the RNA transcripts or the DNA clone induced cytopathic effects and produced infectious progeny. The...
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