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Article

Limited recovery of meiotic spindles in living human oocytes after cooling–rewarming observed using polarized light microscopy

1 Nov 2001

Abstract excerpt

BACKGROUND: Spindles are formed from microtubules and are exquisitely sensitive to changes in temperature. An orientation-independent polarized light microscope, the Polscope, can be used to image spindles in living oocytes allowing analysis of spindle kinetics in the living state. This study examined the effects of cooling on spindle disassembly in living human oocytes and spindle recovery after rewarming....

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PMID: 11679523
DOI: 10.1093/humrep/16.11.2374

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