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[Repair of the splicing defect of thalassemic allele beta IVS-2-654 C-->T in cultured human erythroid cells by antisense RNA].

Zhonghua yi xue za zhi - 1 Nov 1997

Gu X, Gong L, Zhang W

Abstract excerpt

OBJECTIVE: To investigate the repair of the splicing defect of thalassemic allele beta IVS-2-654 C-->T (beta 654) in cultured human adult erythroid cells (hAE) by antisense RNA A2. METHODS: Vectors expressing antisense RNA fragments A2 which targeted against the aberrant splice sites of beta 654 pre-mRNA were introduced into hAE by lipid-mediated DNA-transfection method. Quantitation of beta-globin mRNA by RT-PCR...

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PMID: 9772473

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Related research

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[Repair of the splicing defect of thalassemic allele beta IVS-2-654 C-->T in cultured human erythroid cells by antisense RNA].PMID 9772473Read ↗

Reversal of aberrant splicing of beta-thalassemia allele by antisense RNA in vitro and in vivo.Semantic proximity Read ↗

Repair of thalassemic human β-globin mRNA in mammalian cells by antisense oligonucleotidesSemantic proximity Read ↗

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Amelioration of beta654-thalassemia in mouse model with the knockdown of aberrantly spliced beta-globin mRNA.Semantic proximity Read ↗

Restoration of the balanced alpha/beta-globin gene expression in beta654-thalassemia mice using combined RNAi and antisense RNA approach.Semantic proximity Read ↗

Correction of aberrant pre-mRNA splicing by antisense oligonucleotides in beta-thalassemia Egyptian patients with IVSI-110 mutation.Semantic proximity Read ↗

Repair of a splicing defect in erythroid cells from patients with beta-thalassemia/HbE disorder.Semantic proximity Read ↗

[Expression of human betaIVS- II -nt 654 C --> T allele in HeLa cell].Semantic proximity Read ↗

Restoration of correct splicing in thalassemic pre-mRNA by antisense oligonucleotides.Semantic proximity Read ↗

RNA repair restores hemoglobin expression in IVS2-654 thalassemic mice.Semantic proximity Read ↗

High-level expression of hemoglobin A in human thalassemic erythroid progenitor cells following lentiviral vector delivery of an antisense snRNA.Semantic proximity Read ↗

RNA transcripts of the beta-thalassaemia allele IVS-2-654 C-->T: a small amount of normally processed beta-globin mRNA is still produced from the mutant gene.Semantic proximity Read ↗

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