Article
Oligonucleotide array for detection of common severe determinants of alpha thalassemia.
Journal of biotechnology - 12 Jan 2005
Ye Bang-Ce, Zhang Zhuanfeng, Lei Zhengsong
Abstract excerpt
A simple and an efficient oligonucleotide array was developed to identify common severe determinants of alpha (alpha) thalassemia. A total of 14 probes were designed to detect the most frequently three deletions (-alpha(3.7), -alpha(4.2), -(SEA)) and two non-deletions (alpha(Quong Sze), alpha(Constant Spring)). PCR products were amplified from human genomic DNA and allowed to hybridize with the oligonucleotide...
Topics
- Biomarkers
- China
- DNA Mutational Analysis
- Genetic Predisposition to Disease
- Genetic Testing
- Hemoglobin H
- Humans
- Oligonucleotide Array Sequence Analysis
- Polymorphism, Genetic
- Reproducibility of Results
- Risk Assessment
- Risk Factors
- Sensitivity and Specificity
- Severity of Illness Index
- alpha-Thalassemia