Article
Activation of the gab operon in an RpoS-dependent manner by mutations that truncate the inner core of lipopolysaccharide in Escherichia coli.
Journal of bacteriology - 1 Dec 2004
Joloba Moses L, Clemmer Katy M, Sledjeski Darren D, Rather Philip N
Abstract excerpt
The gab operon (gabDTPC) in Escherichia coli functions in the conversion of gamma-aminobutyrate to succinate. One component of gab operon regulation involves the RpoS sigma factor, which mediates activation at high cell density. Transposon mutagenesis was used to identify new genes that regulate gab operon expression in rich media. A Tn5tmp insertion in the hldD (formerly rfaD) gene increased gabT::lacZ...
Topics
- Bacterial Proteins
- Culture Media
- DNA Transposable Elements
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Lipopolysaccharides
- Mutagenesis, Insertional
- Mutation
- Operon
- Sigma Factor
- gamma-Aminobutyric Acid