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Diagnostic Real-Time PCR for Detection of <i>Salmonella</i> in Food

1 Dec 2004

Abstract excerpt

A robust 5' nuclease (TaqMan) real-time PCR was developed and validated in-house for the specific detection of Salmonella in food. The assay used specifically designed primers and a probe target within the ttrRSBCA locus, which is located near the Salmonella pathogenicity island 2 at centisome 30.5. It is required for tetrathionate respiration in Salmonella. The assay correctly identified all 110 Salmonella...

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PMID: 15574899
DOI: 10.1128/aem.70.12.7046-7052.2004

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