A Multiplex Real-Time Polymerase Chain Reaction for Simultaneous Detection of <i>Salmonella</i> spp., <i>Escherichia coli</i> O157, and <i>Listeria monocytogenes</i> in Meat Products

Abstract excerpt

To achieve an effective detection of Salmonella spp., Escherichia coli O157, and Listeria monocytogenes in meat products, a multiplex real-time polymerase chain reaction (PCR) coupled with a multipathogen enrichment strategy was developed in this study. Pathogen-specific DNA sequences in the invA, rfbE, and hlyA genes were employed to design primers and TaqMan probes for identifying Salmonella spp., E. coli O157,...

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