Article
Visualization of trans-homolog enhancer-promoter interactions at the Abd-B Hox locus in the Drosophila embryo.
Developmental cell - 1 Dec 2004
Ronshaugen Matthew, Levine Mike
Abstract excerpt
The Hox gene Abdominal-B (Abd-B) controls the morphogenesis of posterior abdominal segments in Drosophila. Expression is regulated by a series of 3' enhancers that are themselves transcribed. RNA FISH was used to visualize nascent transcripts associated with coding and noncoding regions of Abd-B in developing embryos. Confocal imaging suggests that distal enhancers often loop to the Abd-B promoter region....
Topics
- Alleles
- Animals
- DNA
- Drosophila Proteins
- Drosophila melanogaster
- Enhancer Elements, Genetic
- Homeodomain Proteins
- Homozygote
- Image Processing, Computer-Assisted
- In Situ Hybridization, Fluorescence
- Microscopy, Confocal
- Models, Biological
- Models, Genetic
- Mutation
- Promoter Regions, Genetic
- Protein Binding
- RNA
- RNA, Messenger