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Article

Establishment by an original single-cell cloning method and characterization of an immortal mouse melanoblast cell line (NCCmelb4).

Pigment cell research - 1 Dec 2004

Ito Masaru, Kawa Yoko, Watabe Hidenori, Ono Hirotake, Ooka Shiho, Nakamura Masayuki, Soma Yoshinao, Mizoguchi Masako

Abstract excerpt

We devised a unique new single-cell cloning method which uses microscope cover glasses and established a melanoblast cell line derived from mouse neural crest cells. A microscope cover glass was nicked and broken into small pieces and put on a dish. Culture medium and a suspension of 20-30 cells/ml were dropped in the dish. After 1-3 d, a piece of glass to which only one cell was adhered was picked up and...

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Identifiers

PMID: 15541022
DOI: 10.1111/j.1600-0749.2004.00184.x

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KIT

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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