Article
The nuclear gene MRS2 is essential for the excision of group II introns from yeast mitochondrial transcripts in vivo.
The Journal of biological chemistry - 5 Apr 1992
Wiesenberger G, Waldherr M, Schweyen R J
Abstract excerpt
RNA splicing defects in mitochondrial intron mutants can be suppressed by a high dosage of several proteins encoded by nuclear genes. In this study we report on the isolation, nucleotide sequence, and possible functions of the nuclear MRS2 gene. When present on high copy number plasmids, the MRS2 gene acts as a suppressor of various mitochondrial intron mutations, suggesting that the MRS2 protein functions as a...
Topics
- Amino Acid Sequence
- Blotting, Northern
- Blotting, Southern
- Cloning, Molecular
- DNA, Fungal
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Genes, Fungal
- Genotype
- Introns