Article
Cloning and characterization of cDNA encoding canine alpha-L-iduronidase. mRNA deficiency in mucopolysaccharidosis I dog.
The Journal of biological chemistry - 5 Apr 1992
Stoltzfus L J, Sosa-Pineda B, Moskowitz S M, Menon K P, Dlott B, Hooper L, Teplow D B, Shull R M, Neufeld E F
Abstract excerpt
alpha-L-Iduronidase is a lysosomal enzyme, the deficiency of which causes mucopolysaccharidosis I (MPS I); a canine MPS I colony has been bred to test therapeutic intervention. The enzyme was purified to apparent homogeneity from canine testis and found to consist of two electrophoretically separ...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Chromosome Mapping
- Chromosomes, Human, Pair 4
- Cloning, Molecular
- DNA
- Disease Models, Animal
- Dogs
- Electrophoresis, Polyacrylamide Gel
- Genetic Vectors
- Glycosylation
- Humans
- Iduronidase