Article
Caprine beta-mannosidase: sequencing and characterization of the cDNA and identification of the molecular defect of caprine beta-mannosidosis.
Genomics - 1 Oct 1996
Leipprandt J R, Kraemer S A, Haithcock B E, Chen H, Dyme J L, Cavanagh K T, Friderici K H, Jones M Z
Abstract excerpt
The complete sequence of the caprine beta-mannosidase cDNA coding region has been determined, and a mutation that is associated with caprine beta-mannosidosis has been identified. Reverse transcriptase-polymerase chain reactions were performed using primers based on bovine and, later, goat cDNA sequences to produce an overlapping series of amplicons covering the entire coding region. The composite cDNA codes for...
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