Article
Substitutions in region 2.4 of sigma70 allow recognition of the sigmaS-dependent aidB promoter.
The Journal of biological chemistry - 31 Dec 2004
Lacour Stephan, Leroy Olivier, Kolb Annie, Landini Paolo
Abstract excerpt
The strict dependence of transcription from the aidB promoter (PaidB) on the Esigma(S) form of RNA polymerase is because of the presence of a C nucleotide as the first residue of the -10 promoter sequence (-12C), which does not allow an open complex formation by Esigma(70). In this report, sigma(70) mutants carrying either the Q437H or the T440I single amino acid substitutions, which allow -12C recognition by...
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