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Article

Alteration of the tertiary structure of the major bee venom allergen Api m 1 by multiple mutations is concomitant with low IgE reactivity.

Protein science : a publication of the Protein Society - 1 Nov 2004

Buhot Cécile, Chenal Alexandre, Sanson Alain, Pouvelle-Moratille Sandra, Gelb Michael H, Ménez André, Gillet Daniel, Maillère Bernard

Abstract excerpt

We have engineered a recombinant form of the major bee venom allergen (Api m 1) with the final goal of reducing its IgE reactivity. This molecule (Api mut) contains 24 mutations and one deletion of 10 amino acids. The successive introduction of these sequence modifications led to a progressive loss of specific IgE and IgG reactivity and did not reveal any immunodominant epitopes. However, Api mut exhibited a...

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Identifiers

PMID: 15459335
DOI: 10.1110/ps.04885404
PMCID: PMC2286571

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Source receipt: pubmed, retrieved 7 Sept 2026.

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