Article
[Use of polymerase chain reaction in medical diagnosis].
Przeglad lekarski - 1 Jan 1999
Swiat A, Stepień E, Andres J, Dziatkowiak A
Abstract excerpt
Principles of the polymerase chain reaction (PCR) and its modifications are discussed: reverse transcription-PCR (RT-PCR), nested PCR, restriction fragment length polymorphism-PCR (RFLP-PCR). Different methods of the PCR products detection used in routine diagnostics and research are presented: electrophoresis and chromatography techniques, immunoenzymatic methods, hybridisation and sequencing. Applications of...
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