Article
[Polymerase chain reaction in clinical diagnosis].
Orvosi hetilap - 1 Apr 1990
Perl A, Fehér J
Abstract excerpt
The polymerase chain reaction (PCR) is a novel method that amplifies selective sequences of DNA or RNA molecules with unparalleled efficiency. The specificity of PCR amplification is based on two oligonucleotide primers which flank the DNA fragment to be multiplied and hybridize to opposite strands. Repeated cycles of thermal denaturation, primer annealing, and enzymatic extension by the heatstable Taq DNA...
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