Article
The dynamics of assembly of a cytoplasmic membrane protein in Escherichia coli.
The Journal of biological chemistry - 15 Mar 1992
Traxler B, Lee C, Boyd D, Beckwith J
Abstract excerpt
The topology of integral cytoplasmic membrane proteins can be analyzed using alkaline phosphatase fusions by determining which constructs have low and which have high specific activity. We show that in all cases the enzymatic activity is due to the fraction of the alkaline phosphatase moiety of t...
Topics
- ATP-Binding Cassette Transporters
- Alkaline Phosphatase
- Carrier Proteins
- Cell Membrane
- Chloramphenicol O-Acetyltransferase
- Endopeptidases
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Genotype
- Maltose-Binding Proteins
- Membrane Proteins
- Models, Biological
- Models, Structural
- Monosaccharide Transport Proteins
- Periplasmic Binding Proteins
- Recombinant Fusion Proteins