Article
Biochemical and histochemical evidence of nonspecific binding of alpha7nAChR antibodies to mouse brain tissue.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society - 1 Oct 2004
Herber Donna L, Severance Emily G, Cuevas Javier, Morgan Dave, Gordon Marcia N
Abstract excerpt
Alpha 7 nicotinic acetylcholine receptors are involved in learning and memory, and are implicated in the pathology of Alzheimer's disease and schizophrenia. Detection of alpha7 subunits can be accomplished via immunodetection or alpha-bungarotoxin-binding techniques. Standard protocols for immunohistochemistry and Western blotting were followed using several commercially available antibodies. Various mice were...
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