Article
Presence of alpha7 nicotinic acetylcholine receptors on dorsal root ganglion neurons proved using knockout mice and selective alpha-neurotoxins in histochemistry.
Journal of neurochemistry - 1 May 2009
Shelukhina Irina V, Kryukova Elena V, Lips Katrin Susanne, Tsetlin Victor I, Kummer Wolfgang
Abstract excerpt
In complex tissues where multiple subtypes of nicotinic acetylcholine receptors (nAChRs) are expressed, immunohistochemistry has been the most popular tool for investigation of nAChR subunit distribution. However, recent studies with nAChR subunit knockout mice demonstrated that a large panel of antibodies is unsuitable. Thus, we aimed to develop a histochemical method for selective labeling of alpha7 nAChR with...
Topics
- Animals
- Bungarotoxins
- Cobra Neurotoxin Proteins
- Female
- Ganglia, Spinal
- Genotype
- Histocytochemistry
- In Vitro Techniques
- Iodine Radioisotopes
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Muscle, Skeletal