Article
[A study on detection method of lamivudine related mutations in hepatitis B virus polymerase gene].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Mar 2004
Ding Jing-juan, Zhang Wei-san, Zhang Li-sha
Abstract excerpt
OBJECTIVE: To establish a simple and accurate method for rapid detection of lamivudine related mutations in hepatitis B virus (HBV) polymerase gene. METHODS: HBV polymerase gene fragments of covering B and C active region were amplified by nested polymerase chain reaction (nPCR) or nested mismatched PCR. The PCR products were digested with Nde I or Nia III and subjected to electrophoresis on agarose gel,...
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