Article
Essential motifs in the 3' untranslated region required for retrotransposition and the precise start of reverse transcription in non-long-terminal-repeat retrotransposon SART1.
Molecular and cellular biology - 1 Sept 2004
Osanai Mizuko, Takahashi Hidekazu, Kojima Kenji K, Hamada Mitsuhiro, Fujiwara Haruhiko
Abstract excerpt
Non-long-terminal-repeat (non-LTR) retrotransposons amplify their copies by reverse transcribing mRNA from the 3' end, but the initial processes of reverse transcription are still unclear. We have shown that a telomere-specific non-LTR retrotransposon of the silkworm, SART1, requires the 3' untranslated region (3' UTR) for retrotransposition. With an in vivo retrotransposition assay, we identified several novel...
Topics
- 3' Untranslated Regions
- Animals
- Base Sequence
- Bombyx
- Cell Line
- Green Fluorescent Proteins
- Luminescent Proteins
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Nucleopolyhedroviruses
- RNA