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Article

Properties of P-glycoprotein with mutations in the "catalytic carboxylate" glutamate residues.

The Journal of biological chemistry - 5 Nov 2004

Tombline Gregory, Bartholomew Lori A, Tyndall Grace A, Gimi Khursheed, Urbatsch Ina L, Senior Alan E

Abstract excerpt

It is known from earlier work that two conserved Glu residues, designated "catalytic carboxylates," are critical for function in P-glycoprotein (Pgp). Here the role of these residues (Glu-552 and Glu-1197 in mouse MDR3 Pgp) was studied further. Mutation E552Q or E1197Q reduced Pgp-ATPase to low but still measurable rates. Two explanations previously offered for effects of these mutations, namely that ADP release...

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PMID: 15326176
DOI: 10.1074/jbc.M408052200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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