Article
Cloning and sequencing of a 1.3 KB variant of human thyrotropin receptor mRNA lacking the transmembrane domain.
Biochemical and biophysical research communications - 16 Sept 1992
Graves P N, Tomer Y, Davies T F
Abstract excerpt
We amplified human thyroidal cDNA using oligonucleotide primers designed to reveal putative human thyrotropin receptor (hTSHR) mRNA variants encoding the extracellular, ligand-binding domain but lacking the transmembrane domain. Whereas the major 4.3 kb hTSHR mRNA species was not amplified to detectable levels, several shorter products were detected. A strongly amplified 1 kb product was cloned and sequenced. It...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Cell Membrane
- Cloning, Molecular
- DNA
- Genetic Variation
- Graves Disease
- Humans
- Molecular Sequence Data
- Nucleic Acid Hybridization