Article
An improved 'cold SSCP' method for the genotypic and subgenotypic characterization of Cryptosporidium.
Molecular and cellular probes - 1 Oct 2004
Gasser Robin B, Abs EL-Osta Youssef G, Prepens Sandra, Chalmers Rachel M
Abstract excerpt
A simple, non-isotopic PCR-based single-strand conformation polymorphism ('cold SSCP') method is described which allows the efficient detection of genetic variation among and within genotypes of Cryptosporidium parvum. This low cost approach has important advantages over other 'genotyping' methods and is applicable to a wide range of genetic loci and organisms.
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