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Survey of Cryptosporidium parvum genotypes in humans from the UK by mutation scanning analysis of a heat shock protein gene region.

Molecular and cellular probes - 1 Aug 2003

El-Osta Youssef G Abs, Chalmers Rachel M, Gasser Robin B

Abstract excerpt

A mutation scanning-selective sequencing approach was employed for the genotypic identification and differentiation of Cryptosporidium parvum isolates. Genomic DNA samples (n=158) from Cryptosporidium oocysts from humans with clinical cryptosporidiosis (following recent foreign travel or during different outbreaks) in the UK were subjected to PCR-based single-strand conformation polymorphism (SSCP) analysis of a...

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PMID: 12944113
DOI: 10.1016/s0890-8508(03)00030-6

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