Article
Genotyping Cryptosporidium parvum with an hsp70 single-nucleotide polymorphism microarray.
Applied and environmental microbiology - 1 Apr 2002
Straub Timothy M, Daly Don S, Wunshel Sharon, Rochelle Paul A, DeLeon Ricardo, Chandler Darrell P
Abstract excerpt
We investigated the application of an oligonucleotide microarray to (i) specifically detect Cryptosporidium spp., (ii) differentiate between closely related C. parvum isolates and Cryptosporidium species, and (iii) differentiate between principle genotypes known to infect humans. A microarray of 68 capture probes targeting seven single-nucleotide polymorphisms (SNPs) within a 190-bp region of the hsp70 gene of...
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