Article
Automated SNP detection in expressed sequence tags: statistical considerations and application to maritime pine sequences.
Plant molecular biology - 1 Feb 2004
Dantec Loïck Le, Chagné David, Pot David, Cantin Olivier, Garnier-Géré Pauline, Bedon Frank, Frigerio Jean-Marc, Chaumeil Philippe, Léger Patrick, Garcia Virginie, Laigret Frédéric, De Daruvar Antoine, Plomion Christophe
Abstract excerpt
We developed an automated pipeline for the detection of single nucleotide polymorphisms (SNPs) in expressed sequence tag (EST) data sets, by combining three DNA sequence analysis programs: Phred, Phrap and PolyBayes. This application requires access to the individual electrophoregram traces. First, a reference set of 65 SNPs was obtained from the sequencing of 30 gametes in 13 maritime pine (Pinus pinaster Ait.)...
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