Article
Development of highly reliable in silico SNP resource and genotyping assay from exome capture and sequencing: an example from black spruce (Picea mariana).
Molecular ecology resources - 1 Mar 2016
Pavy Nathalie, Gagnon France, Deschênes Astrid, Boyle Brian, Beaulieu Jean, Bousquet Jean
Abstract excerpt
Picea mariana is a widely distributed boreal conifer across Canada and the subject of advanced breeding programmes for which population genomics and genomic selection approaches are being developed. Targeted sequencing was achieved after capturing P. mariana exome with probes designed from the sequenced transcriptome of Picea glauca, a distant relative. A high capture efficiency of 75.9% was reached although...
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